bsm 52396r  (Bioss)

Journal: PLOS ONE

Article Title: Inhibition of long non-coding RNA NEAT1 suppressed the epithelial mesenchymal transition through the miR-204-5p/Six1 axis in asthma

doi: 10.1371/journal.pone.0312020

Figure Lengend Snippet: (A-D) The expression levels of E-cadherin (A), N-cadherin (B), vimentin (C), and α-SMA (D) were determined by immunofluorescence; (E) The expression levels of E‑cadherin, vimentin, N-cadherin, and α‑SMA were detected using Western blot. Experiments were triply replicated for robustness. * p <0.05, ** p < 0.01, and *** p <0.001.

Article Snippet: The membranes were incubated overnight at 4°C with the following primary antibodies: E-cadherin (1:1,000, bs-1519R, Bioss, China), N-cadherin (1:1,000, A19083, Abclonal, China), vimentin (1:1,000, A19607, Abclonal, China), α-SMA (1:1,000, bsm-52396R, Bioss, China), and β-actin (1:10,000, EM21002, Huabio, China).

Techniques: Expressing, Immunofluorescence, Western Blot

Journal: Acta Biochimica et Biophysica Sinica

Article Title: Role of Emerin in regulating fibroblast differentiation and migration at the substrate of stiffness coupled topology

doi: 10.3724/abbs.2024094

Figure Lengend Snippet: Differential expressions of α-SMA and Emerin after incubation of HSF with different stiffness-coupled topological substrates (A) Immunofluorescence staining of α-SMA showing nuclei (blue) and α-SMA (green). (B) Statistical analysis of the average fluorescence intensity of α-SMA. (C) Statistical analysis of the mean fluorescence intensity of Emerin. (D) Immunofluorescence staining of Emerin, depicting nuclei (blue), and Emerin (green). The error bars denote the standard deviation. Twenty cells from each group were analyzed using two-way ANOVA, and the experiment was repeated three times.

Article Snippet: The cells were blocked with an immunofluorescence sealing solution (P00102; Beyotime) for 1 h, and then incubated with primary anti-α-SMA antibody (bsm-52396R; Bioss, Beijing, China) for 90 min at room temperature, followed by wash with PBS.

Techniques: Incubation, Immunofluorescence, Staining, Fluorescence, Standard Deviation

Journal: Acta Biochimica et Biophysica Sinica

Article Title: Role of Emerin in regulating fibroblast differentiation and migration at the substrate of stiffness coupled topology

doi: 10.3724/abbs.2024094

Figure Lengend Snippet: Emerin knockdown reduces α-SMA expression in cells (A) Immunofluorescence staining showing α-SMA (green) and nuclei (blue). (B) Statistical analysis of the average fluorescence intensity of α-SMA. The data from each group were analyzed using a t test, with 20 cells from each group selected for fluorescence intensity analysis.

Article Snippet: The cells were blocked with an immunofluorescence sealing solution (P00102; Beyotime) for 1 h, and then incubated with primary anti-α-SMA antibody (bsm-52396R; Bioss, Beijing, China) for 90 min at room temperature, followed by wash with PBS.

Techniques: Knockdown, Expressing, Immunofluorescence, Staining, Fluorescence

Journal: eBioMedicine

Article Title: Akkermansia muciniphila prevents cold-related atrial fibrillation in rats by modulation of TMAO induced cardiac pyroptosis

doi: 10.1016/j.ebiom.2022.104087

Figure Lengend Snippet: Cold exposure induces AF and gut microbiota dysbiosis in rats. (A) Endocardial and surface electrograms recordings in response to burst pacing in RT and Cold rats. ( B ) Number of rats in which AF could be reproducibly induced by right atrial burst pacing. ( C ) AF duration in RT and Cold groups (n=13 per group). (D) Representative images of Masson's trichrome staining and the collagen volume fraction in the atria of RT and Cold rats (n = 3 per group). Magnification × 200, scale bar = 50μm. (E) Representative bands and quantification of expressions of TGF-β1 and α-SMA in rats of RT and Cold rats groups (n = 6 per group). (F) Principal coordinate analysis (PCoA) of unweighted UniFrac revealed clustering of the gut microbiota after temperatures. Each dot represents a single sample of feces. (G) The top 10 of relative abundance of gut microbiota at family level in feces of RT and Cold rats (n = 13 per group). GAPDH was used for normalization. Data are expressed as mean ± SEM and compared by Student's t test ( D and E ) or Wilcoxon test (C) . AF inducibility (B) was presented as numbers and compared by Fisher exact test. RT, room temperature. AF, atrial fibrillation. SR, sinus rhythm.

Article Snippet: Briefly, approximately 30∼50 μg of proteins were separated by electrophoresis on 8∼12% SDS–PAGE and then transferred moist to PVDF membranes (Millipore, Billerica, MA,USA).The primary antibodies were as following: anti-TGF-β1 (Bioss, cat#bs-0103R, RRID: AB_10855750), anti-α-SMA (Bioss, cat#bsm-52396R), anti-BAX (Proteintech, cat#60267-1-Ig, RRID: AB_2848213), anti-Bcl2 (abcom, cat#ab196495), anti-GAPDH (ZSGB-Bio, cat#TA-08, RRID: AB_2747414), anti-β-actin (ZSGB-Bio, cat#TA-09, RRID: AB_2636897), anti-ZO-1 (Proteintech, cat#21773-1-AP, RRID:AB_10733242), anti-Occludin(Abcam, cat#ab167161, RRID: AB_2756463), anti-Claudin-4 (Proteintech, cat#16195-1-AP, RRID: AB_2082969), anti-FMO3 (Proteintech, cat#17469-1-AP, RRID: AB_2878410), anti-Caspas1-p20 (Proteintech, cat#22915-1-AP, RRID: AB_2876874), anti-N-GSDMD (Santa Cruz Biotechnology, cat#sc-393581, RRID: AB_2819179).

Techniques: Staining

Journal: eBioMedicine

Article Title: Akkermansia muciniphila prevents cold-related atrial fibrillation in rats by modulation of TMAO induced cardiac pyroptosis

doi: 10.1016/j.ebiom.2022.104087

Figure Lengend Snippet: Oral supplementation of A. muciniphila protects Cold rats against AF. (A) Endocardial and surface electrograms recordings in response to burst pacing in Cold and Cold + A. muciniphila group. Supplementation with A. muciniphila reduced AF inducibility (B) and duration (C) in Cold rats (n = 10 per group). (D) Representative images of Masson's trichrome staining and the collagen volume fraction in the atria of Cold and Cold+ A. muciniphila rats (n = 4 per group). Magnification × 200, scale bar = 50μm. (E) Representative bands and quantification of expressions of TGF-β1 and α-SMA in rats of Cold and Cold+ A. muciniphila groups (n = 6 per group). AF inducibility (F) and AF duration (G) in Cold (n = 10) and Cold + pasteurised A. muciniphila group (n = 9). GAPDH was used for normalization. Data are expressed as mean ± SEM and compared by Student's t test ( D and E ) or Wilcoxon test (C and G) . AF inducibility (B and F) was presented as numbers and compared by Fisher exact test.

Article Snippet: Briefly, approximately 30∼50 μg of proteins were separated by electrophoresis on 8∼12% SDS–PAGE and then transferred moist to PVDF membranes (Millipore, Billerica, MA,USA).The primary antibodies were as following: anti-TGF-β1 (Bioss, cat#bs-0103R, RRID: AB_10855750), anti-α-SMA (Bioss, cat#bsm-52396R), anti-BAX (Proteintech, cat#60267-1-Ig, RRID: AB_2848213), anti-Bcl2 (abcom, cat#ab196495), anti-GAPDH (ZSGB-Bio, cat#TA-08, RRID: AB_2747414), anti-β-actin (ZSGB-Bio, cat#TA-09, RRID: AB_2636897), anti-ZO-1 (Proteintech, cat#21773-1-AP, RRID:AB_10733242), anti-Occludin(Abcam, cat#ab167161, RRID: AB_2756463), anti-Claudin-4 (Proteintech, cat#16195-1-AP, RRID: AB_2082969), anti-FMO3 (Proteintech, cat#17469-1-AP, RRID: AB_2878410), anti-Caspas1-p20 (Proteintech, cat#22915-1-AP, RRID: AB_2876874), anti-N-GSDMD (Santa Cruz Biotechnology, cat#sc-393581, RRID: AB_2819179).

Techniques: Staining

Journal: eBioMedicine

Article Title: Akkermansia muciniphila prevents cold-related atrial fibrillation in rats by modulation of TMAO induced cardiac pyroptosis

doi: 10.1016/j.ebiom.2022.104087

Figure Lengend Snippet: Caspase1 knockout protects against cold-related AF in mice by restraint of atrial pyroptosis. AF induction rate (A) and AF duration (B) in RT and Cold mice (n=7 per group). (C) Representative bands and quantification of Casp1-p20 and Cleaved-GSDMD in atria of RT and Cold mice (n=6 per group). (D) Endocardial and surface electrograms recordings in response to burst pacing in Casp1 flox/flox and Casp1 mef2c / mef2c mice with a three-week cold exposure (n=7 per group). (E) AF induction rate. (F) AF duration. Representative bands of the protein expressions of Casp1-p20 and Cleaved-GSDMD (G) , TGF-β1 and α-SMA (H) in atrial tissue from Casp1 flox/flox and Casp1 mef2c / mef2c mice (n=6 per group). (I) Quantification of Casp1-p20, Cleaved-GSDMD, TGF-β1 and α-SMA. GAPDH was used for normalization. The data are given as mean ± SEM and compared by Student's t test ( C and I ) or Wilcoxon test ( B and F ). AF inducibility ( A and E ) compared by Fisher exact test.

Article Snippet: Briefly, approximately 30∼50 μg of proteins were separated by electrophoresis on 8∼12% SDS–PAGE and then transferred moist to PVDF membranes (Millipore, Billerica, MA,USA).The primary antibodies were as following: anti-TGF-β1 (Bioss, cat#bs-0103R, RRID: AB_10855750), anti-α-SMA (Bioss, cat#bsm-52396R), anti-BAX (Proteintech, cat#60267-1-Ig, RRID: AB_2848213), anti-Bcl2 (abcom, cat#ab196495), anti-GAPDH (ZSGB-Bio, cat#TA-08, RRID: AB_2747414), anti-β-actin (ZSGB-Bio, cat#TA-09, RRID: AB_2636897), anti-ZO-1 (Proteintech, cat#21773-1-AP, RRID:AB_10733242), anti-Occludin(Abcam, cat#ab167161, RRID: AB_2756463), anti-Claudin-4 (Proteintech, cat#16195-1-AP, RRID: AB_2082969), anti-FMO3 (Proteintech, cat#17469-1-AP, RRID: AB_2878410), anti-Caspas1-p20 (Proteintech, cat#22915-1-AP, RRID: AB_2876874), anti-N-GSDMD (Santa Cruz Biotechnology, cat#sc-393581, RRID: AB_2819179).

Techniques: Knock-Out